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Addgene inc gfp rab11
Gfp Rab11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab11/GFP-Rab11+(Plasmid+%2331738)/pmc12861012-358-5-14
Average 93 stars, based on 26 article reviews
gfp rab11 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Plasmid Preparation:

Article Title: FERARI and cargo adaptors coordinate cargo flow through sorting endosomes
Article Snippet: Alexa Fluor 488–goat anti-rabbit IgG (H + L) (Invitrogen; A-11034) and Alexa Fluor 594–goat anti-mouse IgG (H + L) cross-adsorbed secondary antibodies (Invitrogen; R37121) were used for immuno uorescence. .. DNA and Plasmid sources The following commercially available plasmids were obtained: GFP-VIPAS39 (Sino.Bio; HG22032_ACG), GFP-Rabenosyn-5 (Addgene; 37538), turbo-GFP-SNX1 (Origene; RG201844), GFP-RAB11 (Addgene; 12674). .. The plasmids Px458 GFP (Addgene; 48138) and Px459 Puro (Addgene; 62988) were used for cloning gRNAs. mCherry-SNX1 was created by PCR amplifying mCherry from mCherry-Rab11 construct (Plasmid #55124) and ligating into NotI/PmeI sites of SNX1 vector (Origene; RG201844).

Article Title: Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish
Article Snippet: COS-1 cells (CRL-1650-ATCC) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, GIBCO, 10270-106), 1x sodium pyruvate (EUROCLONE, 11360-039) and 1x penicillin-streptomycin (EUROCLONE, ECB3001D), at 37 °C with 5% CO 2 . .. Subconfluent cells were transfected with plasmids encoding myc- or mCherry-tagged WT and mutant ARF3, EGFP-GalT (Addgene, 11929 ) and GFP-rab11 (Addgene, Plasmid #12674) using FuGENE 6 (Promega, E2691), according to the manufacturer’s instructions. ..

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: The endocytic pathway is of central importance for eukaryotic cells, as it enables uptake of extracellular materials, membrane protein quality control and recycling, as well as modulation of receptor signaling.. While the ATPase p97 (VCP, Cdc48) has been found to be involved in the fusion of early endosomes and endolysosomal degradation, its role in endocytic trafficking is still incompletely characterized.. Here, we identify myoferlin (MYOF), a ferlin family member with functions in membrane trafficking and repair, as a hitherto unknown p97 interactor.

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: .. For the ectopic expression of MYOF-HA (pCDNA3.1-MYOF-HA; Addgene plasmid #22443; gift from William Sessa; Bernatchez et al. , 2007 ), GFP-Rab5WT (pEGFP-Rab5; Addgene plasmid #49888; gift from Marci Scidmore), GFP-Rab5QL (pEGFP-Rab5CA(QL); Addgene plasmid #35140; gift from Sergio Grinstein; Bohdanowicz et al. , 2012 ), GFP-Rab7 (pEGFP-Rab7; gift from Reinhard Jahn; Pavlos et al. , 2010 ), GFP-Rab11 (pEGFP-Rab11; Addgene plasmid #12674; gift from Richard Pagano; Choudhury et al. , 2002 ), and GFP-Rab14 (pEGFP-Rab14; gift from Reinhard Jahn; Pavlos et al. , 2010 ), cells were transfected at 50% confluence using polyethylenimine and analyzed 24 h (immunofluorescence) or 48 h (IP) after transfection. .. For siRNA-mediated depletions, cells were seeded in 12-well plates and transfected with 50 nM siRNA using 1.5 μl Oligofectamine (Thermo Fisher) diluted in 125 μl Opti-MEM per well in a final volume of 1 ml.

Transfection:

Article Title: Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish
Article Snippet: COS-1 cells (CRL-1650-ATCC) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, GIBCO, 10270-106), 1x sodium pyruvate (EUROCLONE, 11360-039) and 1x penicillin-streptomycin (EUROCLONE, ECB3001D), at 37 °C with 5% CO 2 . .. Subconfluent cells were transfected with plasmids encoding myc- or mCherry-tagged WT and mutant ARF3, EGFP-GalT (Addgene, 11929 ) and GFP-rab11 (Addgene, Plasmid #12674) using FuGENE 6 (Promega, E2691), according to the manufacturer’s instructions. ..

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: The endocytic pathway is of central importance for eukaryotic cells, as it enables uptake of extracellular materials, membrane protein quality control and recycling, as well as modulation of receptor signaling.. While the ATPase p97 (VCP, Cdc48) has been found to be involved in the fusion of early endosomes and endolysosomal degradation, its role in endocytic trafficking is still incompletely characterized.. Here, we identify myoferlin (MYOF), a ferlin family member with functions in membrane trafficking and repair, as a hitherto unknown p97 interactor.

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: .. For the ectopic expression of MYOF-HA (pCDNA3.1-MYOF-HA; Addgene plasmid #22443; gift from William Sessa; Bernatchez et al. , 2007 ), GFP-Rab5WT (pEGFP-Rab5; Addgene plasmid #49888; gift from Marci Scidmore), GFP-Rab5QL (pEGFP-Rab5CA(QL); Addgene plasmid #35140; gift from Sergio Grinstein; Bohdanowicz et al. , 2012 ), GFP-Rab7 (pEGFP-Rab7; gift from Reinhard Jahn; Pavlos et al. , 2010 ), GFP-Rab11 (pEGFP-Rab11; Addgene plasmid #12674; gift from Richard Pagano; Choudhury et al. , 2002 ), and GFP-Rab14 (pEGFP-Rab14; gift from Reinhard Jahn; Pavlos et al. , 2010 ), cells were transfected at 50% confluence using polyethylenimine and analyzed 24 h (immunofluorescence) or 48 h (IP) after transfection. .. For siRNA-mediated depletions, cells were seeded in 12-well plates and transfected with 50 nM siRNA using 1.5 μl Oligofectamine (Thermo Fisher) diluted in 125 μl Opti-MEM per well in a final volume of 1 ml.

Mutagenesis:

Article Title: Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish
Article Snippet: COS-1 cells (CRL-1650-ATCC) were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, GIBCO, 10270-106), 1x sodium pyruvate (EUROCLONE, 11360-039) and 1x penicillin-streptomycin (EUROCLONE, ECB3001D), at 37 °C with 5% CO 2 . .. Subconfluent cells were transfected with plasmids encoding myc- or mCherry-tagged WT and mutant ARF3, EGFP-GalT (Addgene, 11929 ) and GFP-rab11 (Addgene, Plasmid #12674) using FuGENE 6 (Promega, E2691), according to the manufacturer’s instructions. ..

other:

Article Title: Dynein-Mediated Trafficking: A New Mechanism of Diabetic Podocytopathy
Article Snippet: GFP-Rab11 , Addgene , 12674.

Expressing:

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: The endocytic pathway is of central importance for eukaryotic cells, as it enables uptake of extracellular materials, membrane protein quality control and recycling, as well as modulation of receptor signaling.. While the ATPase p97 (VCP, Cdc48) has been found to be involved in the fusion of early endosomes and endolysosomal degradation, its role in endocytic trafficking is still incompletely characterized.. Here, we identify myoferlin (MYOF), a ferlin family member with functions in membrane trafficking and repair, as a hitherto unknown p97 interactor.

Article Title: Centrosomal P4.1-associated protein is a novel regulator of ESCRT pathway function during endosome maturation
Article Snippet: .. The vector constructs expressing GFP-Rab5, GFP-Rab11, and mCherry-Rab7, GFP-Rab7-WT and GFP-Rab7-DN were purchased from Addgene. siRNA against HRS, ALIX and TSG101 were purchased from Santa Cruz Biotech. .. Primary antibodies used in this study: rabbit anti-CPAP (Proteintech,11517-1-AP); mouse anti-CPAP (Abnova, H00055835-M01); anti-Rab5 (Proteintech, 20228-1-AP); anti-Rab7 (Proteintech, 55469-1-AP or Santa Cruz, sc-376362); Actin-HRP (Proteintech, 60008-1-Ig); -GFP (Proteintech, 66002-1-Ig or 50430-2-AP); -HRS (Santa Cruz, sc-271455); -TSG101 (Santa Cruz, sc-7964; Proteintech, 28283-1-AP); and -ALIX (Biolegend, 634502), EEA1 (Invitrogen, PA1-0337 or BD biosciences 610456) and CD63 (BD biosciences, 556019).

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: .. For the ectopic expression of MYOF-HA (pCDNA3.1-MYOF-HA; Addgene plasmid #22443; gift from William Sessa; Bernatchez et al. , 2007 ), GFP-Rab5WT (pEGFP-Rab5; Addgene plasmid #49888; gift from Marci Scidmore), GFP-Rab5QL (pEGFP-Rab5CA(QL); Addgene plasmid #35140; gift from Sergio Grinstein; Bohdanowicz et al. , 2012 ), GFP-Rab7 (pEGFP-Rab7; gift from Reinhard Jahn; Pavlos et al. , 2010 ), GFP-Rab11 (pEGFP-Rab11; Addgene plasmid #12674; gift from Richard Pagano; Choudhury et al. , 2002 ), and GFP-Rab14 (pEGFP-Rab14; gift from Reinhard Jahn; Pavlos et al. , 2010 ), cells were transfected at 50% confluence using polyethylenimine and analyzed 24 h (immunofluorescence) or 48 h (IP) after transfection. .. For siRNA-mediated depletions, cells were seeded in 12-well plates and transfected with 50 nM siRNA using 1.5 μl Oligofectamine (Thermo Fisher) diluted in 125 μl Opti-MEM per well in a final volume of 1 ml.

Immunofluorescence:

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: The endocytic pathway is of central importance for eukaryotic cells, as it enables uptake of extracellular materials, membrane protein quality control and recycling, as well as modulation of receptor signaling.. While the ATPase p97 (VCP, Cdc48) has been found to be involved in the fusion of early endosomes and endolysosomal degradation, its role in endocytic trafficking is still incompletely characterized.. Here, we identify myoferlin (MYOF), a ferlin family member with functions in membrane trafficking and repair, as a hitherto unknown p97 interactor.

Article Title: p97/VCP Promotes the Recycling of Endocytic Cargo
Article Snippet: .. For the ectopic expression of MYOF-HA (pCDNA3.1-MYOF-HA; Addgene plasmid #22443; gift from William Sessa; Bernatchez et al. , 2007 ), GFP-Rab5WT (pEGFP-Rab5; Addgene plasmid #49888; gift from Marci Scidmore), GFP-Rab5QL (pEGFP-Rab5CA(QL); Addgene plasmid #35140; gift from Sergio Grinstein; Bohdanowicz et al. , 2012 ), GFP-Rab7 (pEGFP-Rab7; gift from Reinhard Jahn; Pavlos et al. , 2010 ), GFP-Rab11 (pEGFP-Rab11; Addgene plasmid #12674; gift from Richard Pagano; Choudhury et al. , 2002 ), and GFP-Rab14 (pEGFP-Rab14; gift from Reinhard Jahn; Pavlos et al. , 2010 ), cells were transfected at 50% confluence using polyethylenimine and analyzed 24 h (immunofluorescence) or 48 h (IP) after transfection. .. For siRNA-mediated depletions, cells were seeded in 12-well plates and transfected with 50 nM siRNA using 1.5 μl Oligofectamine (Thermo Fisher) diluted in 125 μl Opti-MEM per well in a final volume of 1 ml.

Construct:

Article Title: Centrosomal P4.1-associated protein is a novel regulator of ESCRT pathway function during endosome maturation
Article Snippet: .. The vector constructs expressing GFP-Rab5, GFP-Rab11, and mCherry-Rab7, GFP-Rab7-WT and GFP-Rab7-DN were purchased from Addgene. siRNA against HRS, ALIX and TSG101 were purchased from Santa Cruz Biotech. .. Primary antibodies used in this study: rabbit anti-CPAP (Proteintech,11517-1-AP); mouse anti-CPAP (Abnova, H00055835-M01); anti-Rab5 (Proteintech, 20228-1-AP); anti-Rab7 (Proteintech, 55469-1-AP or Santa Cruz, sc-376362); Actin-HRP (Proteintech, 60008-1-Ig); -GFP (Proteintech, 66002-1-Ig or 50430-2-AP); -HRS (Santa Cruz, sc-271455); -TSG101 (Santa Cruz, sc-7964; Proteintech, 28283-1-AP); and -ALIX (Biolegend, 634502), EEA1 (Invitrogen, PA1-0337 or BD biosciences 610456) and CD63 (BD biosciences, 556019).



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